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osteogenic growth medium cat. no. 05465  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc osteogenic growth medium cat. no. 05465
    Preliminary reverse transcription-quantitative PCR experiment. Gene expression analysis of hMSCs incubated with OGM or LC Arneis and Croatina PRPEs, at 5 and 12 days. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, *** P≤0.001 and **** P≤0.0001. hMSCs, human mesenchymal cells; OGM, <t>osteogenic</t> growth medium; LC, low concentration; PRPEs, polyphenol-rich pomace extracts; iBSP, integrin-binding sialoprotein; BMP2, bone morphogenetic protein 2; Runx2, runt-related transcription factor 2.
    Osteogenic Growth Medium Cat. No. 05465, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/osteogenic+growth+medium+cat%2E+no%2E+05465/pmc07169659-128-9-20?v=STEMCELL+Technologies+Inc
    Average 90 stars, based on 1 article reviews
    osteogenic growth medium cat. no. 05465 - by Bioz Stars, 2026-08
    90/100 stars

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    1) Product Images from "Polyphenols from grape pomace induce osteogenic differentiation in mesenchymal stem cells"

    Article Title: Polyphenols from grape pomace induce osteogenic differentiation in mesenchymal stem cells

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2020.4556

    Preliminary reverse transcription-quantitative PCR experiment. Gene expression analysis of hMSCs incubated with OGM or LC Arneis and Croatina PRPEs, at 5 and 12 days. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, *** P≤0.001 and **** P≤0.0001. hMSCs, human mesenchymal cells; OGM, osteogenic growth medium; LC, low concentration; PRPEs, polyphenol-rich pomace extracts; iBSP, integrin-binding sialoprotein; BMP2, bone morphogenetic protein 2; Runx2, runt-related transcription factor 2.
    Figure Legend Snippet: Preliminary reverse transcription-quantitative PCR experiment. Gene expression analysis of hMSCs incubated with OGM or LC Arneis and Croatina PRPEs, at 5 and 12 days. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, *** P≤0.001 and **** P≤0.0001. hMSCs, human mesenchymal cells; OGM, osteogenic growth medium; LC, low concentration; PRPEs, polyphenol-rich pomace extracts; iBSP, integrin-binding sialoprotein; BMP2, bone morphogenetic protein 2; Runx2, runt-related transcription factor 2.

    Techniques Used: Reverse Transcription, Real-time Polymerase Chain Reaction, Gene Expression, Incubation, Expressing, Control, Concentration Assay, Binding Assay

    Gene expression analysis of Col1a1, SPARC, TIMP1 and MMP1 at the mRNA level. Reverse transcription-quantitative PCR data for Col1a1, SPARC, TIMP1 and MMP1 genes obtained after 48 h and 7 days for untreated and treated groups cultured in OGM or Arneis and Croatina PRPEs. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, ** P≤0.01, *** P≤0.001 and **** P≤0.0001. Col1a1, α 1 type 1 collagen; SPARC, secreted protein acidic and cysteine rich; TIMP1, tissue inhibitor of metalloproteinase 1; MMP1, metalloproteinase 1; OGM, osteogenic growth medium; PRPEs, polyphenol-rich pomace extracts; LC, low concentration; HC, high concentration.
    Figure Legend Snippet: Gene expression analysis of Col1a1, SPARC, TIMP1 and MMP1 at the mRNA level. Reverse transcription-quantitative PCR data for Col1a1, SPARC, TIMP1 and MMP1 genes obtained after 48 h and 7 days for untreated and treated groups cultured in OGM or Arneis and Croatina PRPEs. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, ** P≤0.01, *** P≤0.001 and **** P≤0.0001. Col1a1, α 1 type 1 collagen; SPARC, secreted protein acidic and cysteine rich; TIMP1, tissue inhibitor of metalloproteinase 1; MMP1, metalloproteinase 1; OGM, osteogenic growth medium; PRPEs, polyphenol-rich pomace extracts; LC, low concentration; HC, high concentration.

    Techniques Used: Gene Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Cell Culture, Expressing, Control, Concentration Assay

    Gene expression analysis of Runx2, BMP2, OCN and IBSP at the mRNA level. Reverse transcription-quantitative PCR data for Runx2, BMP2, OCN and IBSP genes obtained after 48 h and 7 days for untreated and treated groups cultured in osteogenic differentiation media or Arneis and Croatina PRPEs. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, ** P≤0.01, ***P≤0.001 and **** P≤0.0001. Runx2, runt-related transcription factor 2; BMP2, bone morphogenetic protein 2; OCN, osteocalcin; IBSP, integrin-binding sialoprotein; PRPEs, polyphenol-rich pomace extracts; LC, low concentration; HC, high concentration.
    Figure Legend Snippet: Gene expression analysis of Runx2, BMP2, OCN and IBSP at the mRNA level. Reverse transcription-quantitative PCR data for Runx2, BMP2, OCN and IBSP genes obtained after 48 h and 7 days for untreated and treated groups cultured in osteogenic differentiation media or Arneis and Croatina PRPEs. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, ** P≤0.01, ***P≤0.001 and **** P≤0.0001. Runx2, runt-related transcription factor 2; BMP2, bone morphogenetic protein 2; OCN, osteocalcin; IBSP, integrin-binding sialoprotein; PRPEs, polyphenol-rich pomace extracts; LC, low concentration; HC, high concentration.

    Techniques Used: Gene Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Cell Culture, Expressing, Control, Binding Assay, Concentration Assay

    Gene expression analysis of RANKL, OPG, RANKL/OPG and MMP1/TIMP1 at the mRNA level. Reverse transcription-quantitative PCR data for RANKL, OPG, RANKL/OPG and MMP1/TIMP1 genes obtained after 48 h and 7 days for untreated and treated groups cultured in osteogenic differentiation media or Arneis and Croatina PRPEs. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, ** P≤0.01, *** P≤0.001 and **** P≤0.0001. RANKL, receptor activator of nuclear factor κ-B ligand; OPG, osteoprotegerin; MMP1, metalloproteinase 1; TIMP1, tissue inhibitor of metalloproteinase 1; PRPEs, polyphenol-rich pomace extracts.
    Figure Legend Snippet: Gene expression analysis of RANKL, OPG, RANKL/OPG and MMP1/TIMP1 at the mRNA level. Reverse transcription-quantitative PCR data for RANKL, OPG, RANKL/OPG and MMP1/TIMP1 genes obtained after 48 h and 7 days for untreated and treated groups cultured in osteogenic differentiation media or Arneis and Croatina PRPEs. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, ** P≤0.01, *** P≤0.001 and **** P≤0.0001. RANKL, receptor activator of nuclear factor κ-B ligand; OPG, osteoprotegerin; MMP1, metalloproteinase 1; TIMP1, tissue inhibitor of metalloproteinase 1; PRPEs, polyphenol-rich pomace extracts.

    Techniques Used: Gene Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Cell Culture, Expressing, Control



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    osteogenic growth medium cat. no. 05465  (STEMCELL Technologies Inc)
    90
    STEMCELL Technologies Inc osteogenic growth medium cat. no. 05465
    Preliminary reverse transcription-quantitative PCR experiment. Gene expression analysis of hMSCs incubated with OGM or LC Arneis and Croatina PRPEs, at 5 and 12 days. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, *** P≤0.001 and **** P≤0.0001. hMSCs, human mesenchymal cells; OGM, <t>osteogenic</t> growth medium; LC, low concentration; PRPEs, polyphenol-rich pomace extracts; iBSP, integrin-binding sialoprotein; BMP2, bone morphogenetic protein 2; Runx2, runt-related transcription factor 2.
    Osteogenic Growth Medium Cat. No. 05465, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/osteogenic+growth+medium+cat%2E+no%2E+05465/pmc07169659-128-9-20?v=STEMCELL+Technologies+Inc
    Average 90 stars, based on 1 article reviews
    osteogenic growth medium cat. no. 05465 - by Bioz Stars, 2026-08
    90/100 stars
    		  Buy from Supplier

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    Preliminary reverse transcription-quantitative PCR experiment. Gene expression analysis of hMSCs incubated with OGM or LC Arneis and Croatina PRPEs, at 5 and 12 days. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, *** P≤0.001 and **** P≤0.0001. hMSCs, human mesenchymal cells; OGM, osteogenic growth medium; LC, low concentration; PRPEs, polyphenol-rich pomace extracts; iBSP, integrin-binding sialoprotein; BMP2, bone morphogenetic protein 2; Runx2, runt-related transcription factor 2.

    Journal: International Journal of Molecular Medicine

    Article Title: Polyphenols from grape pomace induce osteogenic differentiation in mesenchymal stem cells

    doi: 10.3892/ijmm.2020.4556

    Figure Lengend Snippet: Preliminary reverse transcription-quantitative PCR experiment. Gene expression analysis of hMSCs incubated with OGM or LC Arneis and Croatina PRPEs, at 5 and 12 days. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, *** P≤0.001 and **** P≤0.0001. hMSCs, human mesenchymal cells; OGM, osteogenic growth medium; LC, low concentration; PRPEs, polyphenol-rich pomace extracts; iBSP, integrin-binding sialoprotein; BMP2, bone morphogenetic protein 2; Runx2, runt-related transcription factor 2.

    Article Snippet: A further control was obtained by culturing hMSC in osteogenic growth medium (cat. no. 05465, MesenCultTM Osteogenic Diff kit; Human; Stemcell Technologies, Inc.) in the fourth column of the plate.

    Techniques: Reverse Transcription, Real-time Polymerase Chain Reaction, Gene Expression, Incubation, Expressing, Control, Concentration Assay, Binding Assay

    Gene expression analysis of Col1a1, SPARC, TIMP1 and MMP1 at the mRNA level. Reverse transcription-quantitative PCR data for Col1a1, SPARC, TIMP1 and MMP1 genes obtained after 48 h and 7 days for untreated and treated groups cultured in OGM or Arneis and Croatina PRPEs. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, ** P≤0.01, *** P≤0.001 and **** P≤0.0001. Col1a1, α 1 type 1 collagen; SPARC, secreted protein acidic and cysteine rich; TIMP1, tissue inhibitor of metalloproteinase 1; MMP1, metalloproteinase 1; OGM, osteogenic growth medium; PRPEs, polyphenol-rich pomace extracts; LC, low concentration; HC, high concentration.

    Journal: International Journal of Molecular Medicine

    Article Title: Polyphenols from grape pomace induce osteogenic differentiation in mesenchymal stem cells

    doi: 10.3892/ijmm.2020.4556

    Figure Lengend Snippet: Gene expression analysis of Col1a1, SPARC, TIMP1 and MMP1 at the mRNA level. Reverse transcription-quantitative PCR data for Col1a1, SPARC, TIMP1 and MMP1 genes obtained after 48 h and 7 days for untreated and treated groups cultured in OGM or Arneis and Croatina PRPEs. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, ** P≤0.01, *** P≤0.001 and **** P≤0.0001. Col1a1, α 1 type 1 collagen; SPARC, secreted protein acidic and cysteine rich; TIMP1, tissue inhibitor of metalloproteinase 1; MMP1, metalloproteinase 1; OGM, osteogenic growth medium; PRPEs, polyphenol-rich pomace extracts; LC, low concentration; HC, high concentration.

    Article Snippet: A further control was obtained by culturing hMSC in osteogenic growth medium (cat. no. 05465, MesenCultTM Osteogenic Diff kit; Human; Stemcell Technologies, Inc.) in the fourth column of the plate.

    Techniques: Gene Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Cell Culture, Expressing, Control, Concentration Assay

    Gene expression analysis of Runx2, BMP2, OCN and IBSP at the mRNA level. Reverse transcription-quantitative PCR data for Runx2, BMP2, OCN and IBSP genes obtained after 48 h and 7 days for untreated and treated groups cultured in osteogenic differentiation media or Arneis and Croatina PRPEs. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, ** P≤0.01, ***P≤0.001 and **** P≤0.0001. Runx2, runt-related transcription factor 2; BMP2, bone morphogenetic protein 2; OCN, osteocalcin; IBSP, integrin-binding sialoprotein; PRPEs, polyphenol-rich pomace extracts; LC, low concentration; HC, high concentration.

    Journal: International Journal of Molecular Medicine

    Article Title: Polyphenols from grape pomace induce osteogenic differentiation in mesenchymal stem cells

    doi: 10.3892/ijmm.2020.4556

    Figure Lengend Snippet: Gene expression analysis of Runx2, BMP2, OCN and IBSP at the mRNA level. Reverse transcription-quantitative PCR data for Runx2, BMP2, OCN and IBSP genes obtained after 48 h and 7 days for untreated and treated groups cultured in osteogenic differentiation media or Arneis and Croatina PRPEs. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, ** P≤0.01, ***P≤0.001 and **** P≤0.0001. Runx2, runt-related transcription factor 2; BMP2, bone morphogenetic protein 2; OCN, osteocalcin; IBSP, integrin-binding sialoprotein; PRPEs, polyphenol-rich pomace extracts; LC, low concentration; HC, high concentration.

    Article Snippet: A further control was obtained by culturing hMSC in osteogenic growth medium (cat. no. 05465, MesenCultTM Osteogenic Diff kit; Human; Stemcell Technologies, Inc.) in the fourth column of the plate.

    Techniques: Gene Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Cell Culture, Expressing, Control, Binding Assay, Concentration Assay

    Gene expression analysis of RANKL, OPG, RANKL/OPG and MMP1/TIMP1 at the mRNA level. Reverse transcription-quantitative PCR data for RANKL, OPG, RANKL/OPG and MMP1/TIMP1 genes obtained after 48 h and 7 days for untreated and treated groups cultured in osteogenic differentiation media or Arneis and Croatina PRPEs. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, ** P≤0.01, *** P≤0.001 and **** P≤0.0001. RANKL, receptor activator of nuclear factor κ-B ligand; OPG, osteoprotegerin; MMP1, metalloproteinase 1; TIMP1, tissue inhibitor of metalloproteinase 1; PRPEs, polyphenol-rich pomace extracts.

    Journal: International Journal of Molecular Medicine

    Article Title: Polyphenols from grape pomace induce osteogenic differentiation in mesenchymal stem cells

    doi: 10.3892/ijmm.2020.4556

    Figure Lengend Snippet: Gene expression analysis of RANKL, OPG, RANKL/OPG and MMP1/TIMP1 at the mRNA level. Reverse transcription-quantitative PCR data for RANKL, OPG, RANKL/OPG and MMP1/TIMP1 genes obtained after 48 h and 7 days for untreated and treated groups cultured in osteogenic differentiation media or Arneis and Croatina PRPEs. Values are expressed as relative expression, compared with the control group. Data are presented as the mean ± standard error of the mean. * P≤0.05, ** P≤0.01, *** P≤0.001 and **** P≤0.0001. RANKL, receptor activator of nuclear factor κ-B ligand; OPG, osteoprotegerin; MMP1, metalloproteinase 1; TIMP1, tissue inhibitor of metalloproteinase 1; PRPEs, polyphenol-rich pomace extracts.

    Article Snippet: A further control was obtained by culturing hMSC in osteogenic growth medium (cat. no. 05465, MesenCultTM Osteogenic Diff kit; Human; Stemcell Technologies, Inc.) in the fourth column of the plate.

    Techniques: Gene Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Cell Culture, Expressing, Control